histagged tlr4  (R&D Systems)

Journal: Cell reports

Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis

doi: 10.1016/j.celrep.2023.113240

Figure Lengend Snippet: (A and B) Fungistasis assays in which BV-2 cells were pretreated with LPS-RS before activation by (A) Aβ peptides or scrambled control (SC) peptide or (B) Aβ-like peptides derived from the Sap-dependent cleavage of APP and addition of viable C. albicans . (C) Brain fungal burdens of wild-type (WT), TLR4 −/− , and APP −/− mice assessed between days 4 and 10 after i.v. injection of 25,000 viable WT C. albicans cells. (D–F) Schematic diagrams of modified ELISAs assessing binding between Aβ peptides and TLR4 with capture and detecting reagent configurations with and without addition of LPS-RS and aggregate data as indicated. n ≥ 4. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Dunnett’s test for multiple comparison or two-tailed Student’s t test. Data are representative of two independent experiments.

Article Snippet: Plates were blocked with i-Block (2%) for 2 h at 37°C and incubated with a 1/2 serial dilution of Histagged TLR4 (3146-TM, R&D systems, Minneapolis, MN) starting from 50 nM for 2 h at 37°C.

Techniques: Activation Assay, Control, Derivative Assay, Injection, Modification, Binding Assay, Comparison, Two Tailed Test

Journal: Cell reports

Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis

doi: 10.1016/j.celrep.2023.113240

Figure Lengend Snippet:

Article Snippet: Plates were blocked with i-Block (2%) for 2 h at 37°C and incubated with a 1/2 serial dilution of Histagged TLR4 (3146-TM, R&D systems, Minneapolis, MN) starting from 50 nM for 2 h at 37°C.

Techniques: Recombinant, Control, Lysis, Magnetic Beads, Software, Enzyme-linked Immunosorbent Assay